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GM-CSFR alpha Protein, Mouse, Recombinant (His)

(Synonyms: SMDP4, GMR-α, GMR-alpha, GMR, GMCSFR-α, GM-CSF-R-α, GMCSFR-alpha, GM-CSF-R-alpha, GMCSFR, GM-CSF R α Protein, CSF2RY, CSF2RX, CSF2RAY, CSF2RAX, CSF2RA, CSF2R, CDw116, CD116) Copy Product Info

Synonyms: SMDP4, GMR-α, GMR-alpha, GMR, GMCSFR-α, GM-CSF-R-α, GMCSFR-alpha, GM-CSF-R-alpha, GMCSFR, GM-CSF R α Protein, CSF2RY, CSF2RX, CSF2RAY, CSF2RAX, CSF2RA, CSF2R, CDw116, CD116

Catalog No. TMPK-00930 Copy Product Info
In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua. GM-CSFR alpha Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 33.1 kDa and the accession number is Q00941.
Pack SizePriceUSA StockGlobal StockQuantity
5 µg$58-In Stock
10 µg$92-In Stock
20 µg$1487-10 days7-10 days
50 µg$2897-10 days7-10 days
100 µg$487-In Stock
200 µg$8837-10 days7-10 days
500 µg$1,9507-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
1. Immobilized Mouse GM-CSF R alpha, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Mouse GM-CSF, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA (QC Test). 2. Mouse GM-CSF R alpha, His Tag captured on CM5 Chip via Anti-his antibody can bind Mouse GM-CSF, No Tag with an affinity constant of 6.32 nM as determined in SPR assay.
Description
In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua. GM-CSFR alpha Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 33.1 kDa and the accession number is Q00941.
Species
Mouse
Expression System
HEK293 Cells
TagC-His
Accession NumberQ00941
ConstructionLeu30-Pro327
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsSMDP4, GMR-α, GMR-alpha, GMR, GMCSFR-α, GM-CSF-R-α, GMCSFR-alpha, GM-CSF-R-alpha, GMCSFR, GM-CSF R α Protein, CSF2RY, CSF2RX, CSF2RAY, CSF2RAX, CSF2RA, CSF2R, CDw116, CD116
Research Background
In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua.
Chemical Properties
Molecular Weight33.1 kDa (predicted). Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: GM-CSFR alpha Protein, Mouse, Recombinant (His) chemical structure | GM-CSFR alpha Protein, Mouse, Recombinant (His) molecular weight