Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, IHC, FCM

Reactivity: Human

Rabbit IgG

2G215

Human

1. IHC image of TMAH-00841 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
2. Overlay Peak curve showing HepG2 cells surface stained with TMAH-00841 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, IHC, FCM

IHC:1:50-1:200; FCM:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human OCLN

Human

100506658

Signal transduction