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GPA33/A33 Protein, Human, Recombinant (His & Avi)
(Synonyms: MGC129987, MGC129986, GPA33, Glycoprotein A33, A33) Copy Product InfoSynonyms: MGC129987, MGC129986, GPA33, Glycoprotein A33, A33
Catalog No. TMPK-01147 Copy Product Info
GPA33/A33 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 26.5 kDa and the accession number is Q99795-1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $44 | 7-10 days | 7-10 days | |
| 10 µg | $68 | 7-10 days | 7-10 days | |
| 20 µg | $108 | 7-10 days | 7-10 days | |
| 50 µg | $198 | 7-10 days | 7-10 days | |
| 100 µg | $347 | 7-10 days | 7-10 days | |
| 200 µg | $628 | 7-10 days | 7-10 days | |
| 500 µg | $1,400 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1. Immobilized Human GPA33, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-GPA33 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.
2. Anti-GPA33 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human GPA33, His Tag with an affinity constant of 0.69 nM as determined in SPR assay. |
| Description | GPA33/A33 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 26.5 kDa and the accession number is Q99795-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | Q99795-1 |
| Construction | Ile22-Val235 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | MGC129987, MGC129986, GPA33, Glycoprotein A33, A33 |
| Research Background | Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling. |
| Molecular Weight | 26.5 kDa (predicted). Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: GPA33/A33 Protein, Human, Recombinant (His & Avi) chemical structure | GPA33/A33 Protein, Human, Recombinant (His & Avi) molecular weight
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