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GPA33/A33 Protein, Human, Recombinant (His & Avi)

Catalog No. TMPK-01147
Synonyms: A33, Glycoprotein A33, MGC129986, GPA33, MGC129987

GPA33/A33 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 26.5 kDa and the accession number is Q99795-1.

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GPA33/A33 Protein, Human, Recombinant (His & Avi)
Pack Size Availability Price/USD Quantity
100 μg 5 days $ 347.00
500 μg 5 days $ 1,400.00
1 mg 5 days $ 2,330.00
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Biological Description
Technical Params
Product Properties
References and Literature
Description GPA33/A33 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 26.5 kDa and the accession number is Q99795-1.
Species Human
Expression System HEK293
Tag C-His-Avi
Accession Number Q99795-1
Synonyms A33, Glycoprotein A33, MGC129986, GPA33, MGC129987
Construction Recombinant Human GPA33/A33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ile22-Val235.[Accession |Q99795-1]
Protein Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
Endotoxin Less than 1EU per μg by the LAL method.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage

-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Research Background Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.

References and Literature

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Keywords

GPA33/A33 Protein, Human, Recombinant (His & Avi) A33 MGC 129987 MGC-129987 MGC 129986 A-33 GPA 33 Glycoprotein A33 MGC129986 GPA-33 GPA33 A 33 MGC-129986 MGC129987 recombinant recombinant-proteins proteins protein

 

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