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Anti-HAMP Antibody (7O537)

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Catalog No. TMAH-00529
Alias Putative liver tumor regressor, Liver-expressed antimicrobial peptide 1, LEAP1, HEPC

Anti-HAMP Antibody (7O537) is an antibody targeting HAMP. Anti-HAMP Antibody (7O537) can be used in ELISA, WB, IHC.

Anti-HAMP Antibody (7O537)

Anti-HAMP Antibody (7O537)

😃Good
Catalog No. TMAH-00529Alias Putative liver tumor regressor, Liver-expressed antimicrobial peptide 1, LEAP1, HEPC
Anti-HAMP Antibody (7O537) is an antibody targeting HAMP. Anti-HAMP Antibody (7O537) can be used in ELISA, WB, IHC.
Pack SizePriceAvailabilityQuantity
50 μL$207 7-10 days
100 μL$347 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IHC

Reactivity: Human
AliasPutative liver tumor regressor, Liver-expressed antimicrobial peptide 1, LEAP1, HEPC
Ig Type
Rabbit IgG
Clone
7O537
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate
-All lanes: HAMP antibody at 2.15μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 10 KDa
-Observed band size: 25 KDa
2. IHC image of TMAH-00529 diluted at 1:215 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Application
ELISA, WB, IHC
Recommended Dose
WB:1:500-1:5000; IHC:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationSecreted.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundLiver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes. Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human HAMP
Antigen Species
Human
Gene ID
57817
Uniprot ID
Biology Area
Cardiovascular
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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