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Anti-NGF Antibody (7R395)
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Catalog No. TMAH-00816
Alias β-NGF, NGFB, nerve growth factor (β polypeptide), nerve growth factor (beta polypeptide), HSAN5, Beta-NGF
Anti-NGF Antibody (7R395) is an antibody targeting NGF. Anti-NGF Antibody (7R395) can be used in ELISA, WB, IHC.
Anti-NGF Antibody (7R395)
Anti-NGF Antibody (7R395)
😃Good
Catalog No. TMAH-00816Alias β-NGF, NGFB, nerve growth factor (β polypeptide), nerve growth factor (beta polypeptide), HSAN5, Beta-NGF
Anti-NGF Antibody (7R395) is an antibody targeting NGF. Anti-NGF Antibody (7R395) can be used in ELISA, WB, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-NGF Antibody (7R395) is an antibody targeting NGF. Anti-NGF Antibody (7R395) can be used in ELISA, WB, IHC. |
| Alias | β-NGF, NGFB, nerve growth factor (β polypeptide), nerve growth factor (beta polypeptide), HSAN5, Beta-NGF |
| Ig Type | Rabbit IgG |
| Clone | 7R395 |
| Reactivity | Human, Rat |
| Verified Activity | 1. Western Blot -Positive WB detected in: U-87 whole cell lysate, Rat Brain whole cell lysate -All lanes: NGF antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 27 kDa -Observed band size: 39 kDa 2. IHC image of TMAH-00816 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | ELISA, WB, IHC |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Secreted. Endosome lumen. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable). The immature NGF precursor (proNGF) functions as ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI. Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human NGF |
| Antigen Species | Human |
| Gene ID | 4803 |
| Uniprot ID | |
| Biology Area | Neuroscience |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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