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CD7 Protein, Human, Recombinant (His)

(Synonyms: TP41, Tp40, LEU-9, GP40, CD7 molecule) Copy Product Info

Synonyms: TP41, Tp40, LEU-9, GP40, CD7 molecule

Catalog No. TMPY-01195 Copy Product Info
CD7 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 18 kDa and the accession number is P09564.

Batch Information

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Purity:SDS-PAGE: 97.2%; SEC-HPLC: 98.5%
Appearance:Lyophilized powder
Color:White
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Product Information

Bioactivity
Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized human CD7-His at 10 μg/ml (100 μl /well) can bind biotinylated SECTM1-His, The EC50 of biotinylated SECTM1-His is 11-26ng/ml
Description
CD7 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 18 kDa and the accession number is P09564.
Species
Human
Expression System
HEK293 Cells
TagC-His
Accession NumberP09564
ConstructionA DNA sequence encoding the human CD7 (NP_006128.1) extracellular domain (Met 1-Pro 180) was expressed, with a polyhistidine tag at the C-terminus. Predicted N terminal: Ala 26
Protein Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
CD7 Protein, Human, Recombinant (His),CD7 Protein, Human, Recombinant (His)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
SynonymsTP41, Tp40, LEU-9, GP40, CD7 molecule
Research Background
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD7 is a transmembrane protein that is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell / B-cell interaction during early lymphoid development.
Chemical Properties
Molecular Weight18 kDa (predicted); 30-35 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: CD7 Protein, Human, Recombinant (His) chemical structure | CD7 Protein, Human, Recombinant (His) molecular weight