Immobilized Human IL-7 at 0.5 μg/ml (100 μl/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 52.5 ng/ml determined by ELISA.
Human IL-7 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-7, No Tag with an affinity constant of 4.18 nM as determined in SPR assay (Biacore T200).
Measured in a cell proliferation assay using murine 2E8 cells. The ED50 for this effect is 0.1 - 0.5 ng/mL (QC tested).

IL-7 Protein, Human, Recombinant V2 is expressed in E. coli. The accession number is P13232.

Human

E. coli

Interleukin-7,interleukin 7,IL-7

Asp26-His177

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor.