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Anti-SIRT1 Antibody (3M383)
😃Good
Catalog No. TMAY-02042
Alias sirtuin 1, SIR2L1
Anti-SIRT1 Antibody (3M383) is a Mouse antibody targeting SIRT1. Anti-SIRT1 Antibody (3M383) can be used in ELISA,IHC-P,ICC/IF.
Anti-SIRT1 Antibody (3M383)
Anti-SIRT1 Antibody (3M383)
😃Good
Catalog No. TMAY-02042Alias sirtuin 1, SIR2L1
Anti-SIRT1 Antibody (3M383) is a Mouse antibody targeting SIRT1. Anti-SIRT1 Antibody (3M383) can be used in ELISA,IHC-P,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-SIRT1 Antibody (3M383) is a Mouse antibody targeting SIRT1. Anti-SIRT1 Antibody (3M383) can be used in ELISA,IHC-P,ICC/IF. |
| Alias | sirtuin 1, SIR2L1 |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 3M383 |
| Reactivity | Human |
| Verified Activity | 1. Immunochemical staining of human SIRT1 in human lung cancer with mouse monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections). 2. Immunofluorescence staining of SIRT1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human SIRT1 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green). |
| Application | ELISA,IHC-P,ICC/IF |
| Recommended Dose | ELISA: 1:1000-1:2000; IHC-P: 1:500-1:2000; ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SIRT1 (rh SIRT1; TMPY-01869; NP_036370.2; Met 193-Ser 747). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | SIRT1 belongs to the sirtuin family. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT1 is included in class I of the sirtuin family. It is a NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. It deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. SIRT1 also deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. It is involved in HES1- and HEY2-mediated transcriptional repression. SIRT1 inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. It may serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. It also deacetylates 'Lys-16' of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human SIRT1 protein (TMPY-01869) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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