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Anti-CD55/DAF Antibody (7N765)
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Catalog No. TMAY-00258
Alias GPI-DAF, Daf-GPI, Daf1, Daf, CD55 molecule, decay accelerating factor for complement (Cromer blood group)
Anti-CD55/DAF Antibody (7N765) is a Rabbit antibody targeting CD55/DAF. Anti-CD55/DAF Antibody (7N765) can be used in ELISA,ELISA(Cap),FCM,ICC/IF.
Anti-CD55/DAF Antibody (7N765)
Anti-CD55/DAF Antibody (7N765)
😃Good
Catalog No. TMAY-00258Alias GPI-DAF, Daf-GPI, Daf1, Daf, CD55 molecule, decay accelerating factor for complement (Cromer blood group)
Anti-CD55/DAF Antibody (7N765) is a Rabbit antibody targeting CD55/DAF. Anti-CD55/DAF Antibody (7N765) can be used in ELISA,ELISA(Cap),FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $93 | 7-10 days | |
| 100 μL | $293 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: ELISA, ELISA(Cap), FCM, ICC/IF Reactivity: Human |
| Alias | GPI-DAF, Daf-GPI, Daf1, Daf, CD55 molecule, decay accelerating factor for complement (Cromer blood group) |
| Ig Type | Rabbit IgG |
| Clone | 7N765 |
| Reactivity | Human |
| Verified Activity | 1. Flow cytometric analysis of human CD55 expression on K562 cells. Cells were stained with purified anti-Human CD55, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells. 2. Immunofluorescence staining of Human CD55 in JURKAT cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human CD55 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane. |
| Application | ELISA,ELISA(Cap),FCM,ICC/IF |
| Recommended Dose | ELISA: 1:5000-1:10000; ICC-IF: 1:10-1:40; FCM: 1:25-1:100; ELISA(Cap): 1:250-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human CD55 / DAF (rh CD55; TMPY-01292; NP_000565.1; Met 1-Ser 353). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | CD55, also well known as decay-accelerating factor (DAF), is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. It is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. CD55 is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours. DAF seems to exert different functions beyond its immunological role such as promotion of tumorigenesis, decrease of complement mediated tumor cell lysis, autocrine loops for cell rescue and evasion of apoptosis, neoangiogenesis, invasiveness, cell motility. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection. This 70-75 kDa glycoprotein CD55 containing four SCR modules is involved in the regulation of the complement cascade. It inhibits complement activation by suppressing the function of C3/C5 convertases, thereby limiting local generation or deposition of C3a/C5a and membrane attack complex (MAC or C5b-9) production. DAF has been identified as a ligand for an activation-associated, seven-transmembrane lymphocyte receptor, CD97, which is a receptor mediating attachment and infection of several viruses and bacteria. In addition, it has been shown that DAF regulates the interplay between complement and T cell immunity in vivo, and thus may be implicated in immune and tumor biology. |
| Conjucates | Unconjugated |
| Others Formats | APC/PerCP/FITC/PE |
| Antibody Types Available | 5 |
| Immunogen | Recombinant Protein: Human CD55 protein (TMPY-01292) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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