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TOPBioactivityRelated Conjugates and FormulationsAntigen DetailsChemical infoStorage&SolubilityCalculator

Anti-Phospho-YAP1 (Ser127) Antibody (8Q811)

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Catalog No. TMAH-00977
Alias YAP1 (p-Ser127), YAP1 (p-S127), p-YAP1 (Ser127), p-YAP1 (S127), Phospho-YAP1 (S127)

Anti-Phospho-YAP1 (Ser127) Antibody (8Q811) is an antibody targeting Phospho-YAP1 (Ser127). Anti-Phospho-YAP1 (Ser127) Antibody (8Q811) can be used in ELISA, WB, IHC.

Anti-Phospho-YAP1 (Ser127) Antibody (8Q811)

Anti-Phospho-YAP1 (Ser127) Antibody (8Q811)

😃Good
Catalog No. TMAH-00977Alias YAP1 (p-Ser127), YAP1 (p-S127), p-YAP1 (Ser127), p-YAP1 (S127), Phospho-YAP1 (S127)
Anti-Phospho-YAP1 (Ser127) Antibody (8Q811) is an antibody targeting Phospho-YAP1 (Ser127). Anti-Phospho-YAP1 (Ser127) Antibody (8Q811) can be used in ELISA, WB, IHC.
Pack SizePriceAvailabilityQuantity
50 μL$208 7-10 days
100 μL$349 7-10 days
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Instruction Manual of Detection Antibody
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IHC

Reactivity: Human
AliasYAP1 (p-Ser127), YAP1 (p-S127), p-YAP1 (Ser127), p-YAP1 (S127), Phospho-YAP1 (S127)
Ig Type
Rabbit IgG
Clone
8Q811
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in HepG2 whole cell lysate(treated with Calyculin A or not)
-All lanes Phospho-YAP1 antibody at 0.83μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 65 KDa
-Observed band size: 65 KDa
2. IHC image of TMAH-00977 diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Application
ELISA, WB, IHC
Recommended Dose
WB:1:500-1:5000; IHC:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCytoplasm. Nucleus.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundTranscriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization. Plays a key role in controlling cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Suppresses ciliogenesis via acting as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1. In conjunction with WWTR1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation. Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3). Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human Phospho-YAP1 (S127)
Antigen Species
Human
Gene ID
10413
Uniprot ID
Biology Area
Signal Transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Yes-associated protein YAP65 homologYes-associated protein 1YAP65YAP1 (pSer127)YAP1 (pS127)YAP1 (phospho-Ser127)YAP1 (phospho-S127)YAP1YAP 65YAP 1Transcriptional coactivator YAP1pYAP1 (Ser127)pYAP1 (S127)Protein yorkie homolog
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