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DPP4/CD26 Protein, Human, Recombinant (hFc)

(Synonyms: TP103, DPPIV, dipeptidylpeptidase 4, CD26, ADCP2, ADABP) Copy Product Info

Synonyms: TP103, DPPIV, dipeptidylpeptidase 4, CD26, ADCP2, ADABP

Catalog No. TMPY-00778 Copy Product Info
DPP4/CD26 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 112 kDa and the accession number is P27487.

Batch Information

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Purity:92.9%
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
1. Measured by its ability to bind recombinant Cynomolgus CXCL12 in a functional ELISA. 2. Measured by its ability to bind recombinant Human SDF1b in a functional ELISA. 3. Loaded Fc-DPPIV on ProA Biosensor, can bind MERS-CoV Spike/RBD Protein fragment (RBD, aa 367-606, His Tag) with an affinity constant of 1.04 nM as determined by Octet RED System. 4. Loaded Fc-DPPIV on ProA Biosensor, can bind MERS-CoV Spike/S1 Protein (S1 Subunit, aa 1-725, His Tag) with an affinity constant of 0.02 nM as determined by Octet RED System. 5. Loaded Fc-DPPIV on ProA Biosensor, can bind MERS-CoV Spike/RBD Protein fragment (RBD, aa 367-606, His Tag) with an affinity constant of 1.04 nM as determined by Octet RED System. 6. Loaded Fc-DPPIV on ProA Biosensor, can bind MERS-CoV Spike/S1 Protein (S1 Subunit, aa 1-725, His Tag) with an affinity constant of 0.02 nM as determined by Octet RED System.
DPP4/CD26 Protein, Human, Recombinant (hFc)DPP4/CD26 Protein, Human, Recombinant (hFc)
Description
DPP4/CD26 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 112 kDa and the accession number is P27487.
Species
Human
Expression System
HEK293 Cells
TagN-hFc
Accession NumberP27487
ConstructionA DNA sequence encoding the extracellular domain (Asn 29-Pro 766) of the mature form of human DPPIV (NP_001926.2) was expressed with the fused Fc region of human IgG1 at the N-terminus. Predicted N terminal: Glu 20
Protein Purity
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
DPP4/CD26 Protein, Human, Recombinant (hFc)DPP4/CD26 Protein, Human, Recombinant (hFc)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsTP103, DPPIV, dipeptidylpeptidase 4, CD26, ADCP2, ADABP
Research Background
Chemical Properties
Molecular Weight112 kDa (predicted); 120-130 kDa (reducing condition, due to glycosylation)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: DPP4/CD26 Protein, Human, Recombinant (hFc) chemical structure | DPP4/CD26 Protein, Human, Recombinant (hFc) molecular weight