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Anti-Osteopontin Antibody (5D926)
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Catalog No. TMAH-01129
Alias secreted phosphoprotein 1, OPN, ETA-1, BSPI, BNSP
Anti-Osteopontin Antibody (5D926) is an antibody targeting Osteopontin. Anti-Osteopontin Antibody (5D926) can be used in ELISA, WB, IHC.
Anti-Osteopontin Antibody (5D926)
Anti-Osteopontin Antibody (5D926)
😃Good
Catalog No. TMAH-01129Alias secreted phosphoprotein 1, OPN, ETA-1, BSPI, BNSP
Anti-Osteopontin Antibody (5D926) is an antibody targeting Osteopontin. Anti-Osteopontin Antibody (5D926) can be used in ELISA, WB, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC Reactivity: Human |
| Alias | secreted phosphoprotein 1, OPN, ETA-1, BSPI, BNSP |
| Ig Type | Rabbit IgG |
| Clone | 5D926 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: 293T whole cell lysate, U-251 whole cell lysate, U-87 whole cell lysate -All lanes: Osteopontin Antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 36, 34, 33, 34, 34 kDa -Observed band size: 36 kDa 2. IHC image of TMAH-01129 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | ELISA, WB, IHC |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Secreted. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Osteopontin |
| Antigen Species | Human |
| Gene ID | 6696 |
| Uniprot ID | |
| Biology Area | Cancer, Cardiovascular, Signal transduction, Stem cells |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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