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SARS-CoV-2 Spike S1 Protein (HEK293, His)

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Synonyms:

Catalog No. TMPY-05661 Copy Product Info
SARS-CoV-2 Spike S1 Protein (HEK293, His) is expressed in HEK293 mammalian cells with His tag. The accession number is A0A6G7K2L4.

Batch Information

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Purity:> 90 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Description
SARS-CoV-2 Spike S1 Protein (HEK293, His) is expressed in HEK293 mammalian cells with His tag. The accession number is A0A6G7K2L4.
Expression System
HEK293 Cells
TagC-His
Accession NumberA0A6G7K2L4
ConstructionA DNA sequence encoding the SARS-CoV-2 (2019-nCoV) spike protein S1 Subunit (YP_009724390.1) (Val16-Arg685) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Val16
Protein Purity
> 90 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background
Chemical Properties
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: SARS-CoV-2 Spike S1 Protein (HEK293, His) chemical structure