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BDCA-2 Protein, Human, Recombinant (His & Avi), Biotinylated

(Synonyms: HECL, DLEC, CLECSF7, CLECSF11, CLEC4C, CD303, BDCA-2, BDCA2) Copy Product Info

Synonyms: HECL, DLEC, CLECSF7, CLECSF11, CLEC4C, CD303, BDCA-2, BDCA2

Catalog No. TMPK-01118 Copy Product Info
BDCA-2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 22.7 kDa and the accession number is Q8WTT0-1.

Batch Information

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Purity:> 83%
Appearance:Solution
Color:Transparent
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Product Introduction

Bioactivity
Bioactivity
Immobilized Biotinylated Human BDCA-2, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
Description
BDCA-2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 22.7 kDa and the accession number is Q8WTT0-1.
Species
Human
Expression System
HEK293 Cells
TagN-His-Avi
Accession NumberQ8WTT0-1
ConstructionPhe46-Ile213
Protein Purity
> 95% as determined by Tris-Bis PAGE
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationSupplied as 0.22 μm filtered solution in 20 mM Tris, 300 mM NaCl, 200 mM L-arginine (pH 8.5).
SynonymsHECL, DLEC, CLECSF7, CLECSF11, CLEC4C, CD303, BDCA-2, BDCA2
Research Background
BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c() CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c() CD11c(bright) CD123(dim), or on any other cells in blood.BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, BDCA-2 is rapidly internalized at 37 degrees C after mAb labeling.
Chemical Properties
Molecular Weight22.7 kDa (predicted). Due to glycosylation, the protein migrates to 33-40 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
ShippingProteins are shipped with blue ice.
StorageIt is recommended to store the product under sterile conditions at -70°C or lower. Samples are stable for up to 12 months at -80°C. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: BDCA-2 Protein, Human, Recombinant (His & Avi), Biotinylated chemical structure | BDCA-2 Protein, Human, Recombinant (His & Avi), Biotinylated molecular weight