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CD45 Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPK-00424
Synonyms: LCA, LY5, EC 3.1.3.48, CD45, L-CA, T200, CD45R, PTPRC, B220, GP180

PTPRC (also known as CD45),T cells require the protein tyrosine phosphatase CD45 to detect and respond to antigen because it activates the Src family kinase Lck, which phosphorylates the T cell antigen receptor (TCR) complex. CD45 ativates Lck by opposing the negative regulatory kinase Csk. Paradoxically, CD45 has also been implicated in suppressing TCR signaling by dephosphorylating the same signaling motifs within the TCR complex upon which Lck acts. CD45 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 47.3 kDa and the accession number is P08575-4.

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CD45 Protein, Human, Recombinant (His & Avi), Biotinylated
Pack Size Availability Price/USD Quantity
100 μg 5 days $ 814.00
500 μg 5 days $ 3,250.00
1 mg 5 days $ 5,440.00
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Biological Description
Technical Params
Product Properties
References and Literature
Description PTPRC (also known as CD45),T cells require the protein tyrosine phosphatase CD45 to detect and respond to antigen because it activates the Src family kinase Lck, which phosphorylates the T cell antigen receptor (TCR) complex. CD45 ativates Lck by opposing the negative regulatory kinase Csk. Paradoxically, CD45 has also been implicated in suppressing TCR signaling by dephosphorylating the same signaling motifs within the TCR complex upon which Lck acts. CD45 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 47.3 kDa and the accession number is P08575-4.
Species Human
Expression System HEK293 Cells
Tag C-His-Avi
Accession Number P08575-4
Synonyms LCA, LY5, EC 3.1.3.48, CD45, L-CA, T200, CD45R, PTPRC, B220, GP180
Construction Gln26­-Lys416
Protein Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight 47.3 kDa (predicted). Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
Endotoxin < 1 EU/μg by the LAL method.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice.

Research Background PTPRC (also known as CD45),T cells require the protein tyrosine phosphatase CD45 to detect and respond to antigen because it activates the Src family kinase Lck, which phosphorylates the T cell antigen receptor (TCR) complex. CD45 ativates Lck by opposing the negative regulatory kinase Csk. Paradoxically, CD45 has also been implicated in suppressing TCR signaling by dephosphorylating the same signaling motifs within the TCR complex upon which Lck acts.

References and Literature

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Keywords

CD45 Protein, Human, Recombinant (His & Avi), Biotinylated T 200 CD 45 B-220 LCA LY 5 LY5 T-200 EC 3.1.3.48 CD45 GP 180 L-CA CD-45 B 220 T200 CD45R LY-5 PTPRC B220 GP180 GP-180 recombinant recombinant-proteins proteins protein

 

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