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Anti-C-Kit Antibody (3B930)
😃Good
Catalog No. TMAY-03341
Alias v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, SCFR, PBT, C-Kit, CD117
Anti-C-Kit Antibody (3B930) is a Rabbit antibody targeting C-Kit. Anti-C-Kit Antibody (3B930) can be used in WB,ELISA,IP.
Anti-C-Kit Antibody (3B930)
Anti-C-Kit Antibody (3B930)
😃Good
Catalog No. TMAY-03341Alias v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, SCFR, PBT, C-Kit, CD117
Anti-C-Kit Antibody (3B930) is a Rabbit antibody targeting C-Kit. Anti-C-Kit Antibody (3B930) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, ELISA, IP Reactivity: Mouse |
| Alias | v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, SCFR, PBT, C-Kit, CD117 |
| Ig Type | Rabbit IgG |
| Clone | 3B930 |
| Reactivity | Mouse |
| Verified Activity | 1. Anti-KIT rabbit monoclonal antibody at 1:500 dilution. -Lane A: MOLT4 Whole Cell Lysate. -Lane B: Jurkat Whole Cell Lysate. -Lane C: K562 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:110 kDa. -Observed band size:110 kDa(We are unsure as to the identity of these extra bands.) 2. Mouse KIT was immunoprecipitated using: -Lane A:0.5 mg K562 Whole Cell Lysate. -1 µL anti-Mouse KIT rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-Mouse KIT rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 110 kDa. -Observed band size: 110 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:1000; ELISA: 1:25000-1:50000; IP: 0.2-1 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse KIT / c-KIT / CD117 (rM KIT / c-KIT / CD117; TMPY-01802; NP_001116205.1; Met1-Thr523). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family, and CSF-1/PDGF receptor subfamily. C-Kit has tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumor in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumors and in distinguishing seminomas from embryonal carcinomas. Mutations in the c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Mouse KIT / c-KIT / CD117 Protein (TMPY-01802) |
| Antigen Species | Mouse |
| Biology Area | Cancer Drug Targets, Cardiac Stem Cell Markers, Receptor Tyrosine Kinases (RTKs) |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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