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Anti-Phospho-LATS1/2 (Thr1079, 1041) Polyclonal Antibody
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Catalog No. TMAB-01450
Anti-Phospho-LATS1/2 (Thr1079, 1041) Polyclonal Antibody is a Rabbit antibody targeting Phospho-LATS1/2 (Thr1079, 1041). Anti-Phospho-LATS1/2 (Thr1079, 1041) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Anti-Phospho-LATS1/2 (Thr1079, 1041) Polyclonal Antibody
Anti-Phospho-LATS1/2 (Thr1079, 1041) Polyclonal Antibody
😃Good
Catalog No. TMAB-01450
Anti-Phospho-LATS1/2 (Thr1079, 1041) Polyclonal Antibody is a Rabbit antibody targeting Phospho-LATS1/2 (Thr1079, 1041). Anti-Phospho-LATS1/2 (Thr1079, 1041) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF,ELISA Reactivity: Human,Mouse,Rabbit (predicted:Rat,Chicken,Dog,Pig,Cow,Horse) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rabbit (predicted:Rat,Chicken,Dog,Pig,Cow,Horse) |
| Verified Activity | 1. phosphopeptide non phosphopeptide 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-LATS1+LATS2 (Thr1079 +Thr1041)) Polyclonal Antibody, Unconjugated (TMAB-01450) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Sample: Hela (Human) Cell Lysate at 30 μg Primary: Anti-phospho-LATS1+LATS2 (Thr1079 +Thr1041) (TMAB-01450) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kDa Observed band size: 124 kDa 4. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-phospho-LATS1+LATS2 (Thr1079 +Thr1041) (TMAB-01450) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kDa Observed band size: 124 kDa |
| Application | WB,IHC-P,IHC-Fr,IF,ELISA |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton, centrosome. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthesised phosphopeptide: human LATS1 around the phosphorylation site of Thr1079 |
| Antigen Species | Human |
| Gene ID | 26524 |
| Uniprot ID | |
| Biology Area | Tumor suppressors,Kinases,Cytokinesis,Cdk Regulators |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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