IL-6R Protein, Human, Recombinant (hFc)

Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (SIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a SIL-6R protein can mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signaling. Through this so-called 'trans-signaling' mechanism, IL-6 can stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and SIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Pack Size	Availability	Price/USD	Quantity
100 μg	In stock	$ 212.00

Pack Size

Availability

Price/USD

Quantity

100 μg

In stock

$ 212.00

Biological Information

1.Measured by its ability to enhance the activity of IL6 on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 30-125 ng/mL in the presence of 100 ng/mL of recombinant human IL6. 2.Loaded Recombinant Human IL-6R Protein, hFc Tag on ProA Biosensor, can bind Recombinant Human IL-6 Protein, no Tag with an affinity constant of 0.51µM as determined in BLI assay (Routinely tested). 3. Immobilized Recombinant Human IL6 / Interleukin-6 Protein at 2μg/mL (100μL/well) can bind Recombinant Human IL-6R / CD126 Protein (ECD, Fc Tag) , the EC50 is 5-15 ng/mL.

Description

Species	Human
Expression System	HEK293
Tag	hFc
Accession Number	A0N0L5
Synonyms	IL-6R-1, CD126, gp80, IL6Q, IL6RQ, IL-6R, IL-6RA, IL6RA, interleukin 6 receptor
Construction	A DNA sequence encoding the human IL6R (NP_000556.1) (Met1-Pro365) was expressed with the Fc region of human IgG1 at the C-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE.
Molecular Weight	Approxiamtely 65.3 kDa

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (SIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a SIL-6R protein can mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signaling. Through this so-called 'trans-signaling' mechanism, IL-6 can stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and SIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy