Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, ICC, IF, FCM

Reactivity: Human, Mouse

Rabbit IgG

4I767

Human, Mouse

1. Western Blot
-Positive WB detected in Mouse brain tissue,Mouse heart tissue
-All lanes Di-methyl-Histone H3.1(K4)antibody at 0.55μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 15 KDa
-Observed band size: 15 KDa
2. Immunocytochemistry analysis of TMAH-00346 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
3. Immunofluorescence staining of Hela cells with TMAH-00346 at 1:34,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
4. Overlay histogram showing Hela cells stained with TMAH-00346 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, ICC, IF, FCM

WB:1:500-1:2000; ICC:1:50-1:500; IF:1:30-1:200.

Monoclonal

Unconjugated

A synthetic peptide

Human

8350

Epigenetics and Nuclear Signaling