Vinculin Protein, Human, Recombinant (His)

Name: Vinculin Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-02548
Rating: 4.5 (1 reviews)

(Synonyms: vinculin, MVCL, MV, HEL114, CMH15, CMD1W) Copy Product Info

Synonyms: vinculin, MVCL, MV, HEL114, CMH15, CMD1W

Catalog No. TMPY-02548 Copy Product Info

Vinculin Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 118 kDa and the accession number is P18206-2.

Pack Size	Price	USA Stock	Global Stock
5 µg	$108	7-10 days	7-10 days
10 µg	$178	7-10 days	7-10 days
20 µg	$296	7-10 days	7-10 days
50 µg	$696	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized Recombinant Human VCL/Vinculin Protein (His Tag) (Cat#TMPY-02548) at 1 μg/mL (100 μL/well) can bind Vinculin Antibody, Rabbit PAb, Antigen Affinity Purified, the EC50 is 1.0-3.0 ng/mL(QC tested).
Description	Vinculin Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 118 kDa and the accession number is P18206-2.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P18206-2
Construction	A DNA sequence encoding the human VCL (P18206-2) (Met 1-Gln 1066) was expressed, fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Met 1
Protein Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	vinculin, MVCL, MV, HEL114, CMH15, CMD1W
Research Background	Vinculin (VCL) is a cytoskeletal protein that is closely related to both cell-matrix interactions and cell-cell junctions. VCL is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment. This protein has multi-ligand properties and has been found to interact with a number of microfilament associated proteins, such as talin, a-actinin, and paxillin, which reportedly bind to either the head or tail domains of vinculin.

Chemical Properties

Molecular Weight

118 kDa (predicted); 115 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.