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PVRIG Protein, Human, Recombinant (mFc)

TargetMol | SPR
Catalog No. TMPY-06277

PVRIG Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 49.6 kDa and the accession number is Q6DKI7-1.

PVRIG Protein, Human, Recombinant (mFc)

PVRIG Protein, Human, Recombinant (mFc)

TargetMol | SPR
Catalog No. TMPY-06277
PVRIG Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 49.6 kDa and the accession number is Q6DKI7-1.
Pack SizePriceAvailabilityQuantity
100 μg$6007-10 days
1 mg$3,9107-10 days
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Product Information

Biological Activity
Immobilized Human PVRIG, mFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-PVRIG Antibody, hFc Tag with the EC50 of 8.0 ng/ml determined by ELISA.Human PVRIG, mFc Tag captured on CM5 Chip via Anti-Mouse Antibody can bind Human Nectin-2, His Tag with an affinity constant of 0.349 μM as determined in SPR assay (Biacore T200).
Description
PVRIG Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 49.6 kDa and the accession number is Q6DKI7-1.
Species
Human
Expression System
HEK293 Cells
TagC-mFc
Accession NumberQ6DKI7-1
Synonyms
RMO1,RalGDS/AF-6,PREL-2,PREL2,LPD,C7orf15,ALS2CR9,ALS2CR18
Construction
Thr41-Asp171
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight39.6 kDa (Predicted); 50-65 kDa (Due to glycosylation)
EndotoxinLess than 1EU per μg by the LAL method.
FormulationLyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background
Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family.PVRIG is a cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding.

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