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Anti-Flag Tag Antibody-HRP (3S989)

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Catalog No. TMAS-00123H
Alias flagtag, DYKDDDDK-tag

Anti-Flag Tag Antibody-HRP (3S989) is a Rabbit antibody targeting Flag Tag. Anti-Flag Tag Antibody-HRP (3S989) can be used in ELISA, WB.

Anti-Flag Tag Antibody-HRP (3S989)

Anti-Flag Tag Antibody-HRP (3S989)

😃Good
Catalog No. TMAS-00123HAlias flagtag, DYKDDDDK-tag
Anti-Flag Tag Antibody-HRP (3S989) is a Rabbit antibody targeting Flag Tag. Anti-Flag Tag Antibody-HRP (3S989) can be used in ELISA, WB.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
40 μg$6097-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Flag Tag Antibody-HRP (3S989) is a Rabbit antibody targeting Flag Tag. Anti-Flag Tag Antibody-HRP (3S989) can be used in ELISA, WB.
Synonymsflagtag, DYKDDDDK-tag
Ig Type
IgG
Clone
3S989
Specificity
DYKDDDDK tags placed at C-terminal, N-terminal and internal regions of DYKDDDDK fusion proteins
Verified Activity
1. Anti-Flag Tag Antibody-HRP (3S989) (TMAS-00123H) binds with DYKDDDDK Tag.
Coating antigen: DYKDDDDK-tagged fusion protein, 1 µg/ml.
Anti-Flag Tag Antibody-HRP (3S989) (TMAS-00123H) dilution start from 1,000 ng/ml.
EC50= 20.21 ng/ml.
2. Sensitivity and specificity of Anti-Flag Tag Antibody-HRP (3S989) (TMAS-00123H) to C-DYKDDDDK-tag Fusion Protein by Western blot.
Lane 1 : C-terminal DYKDDDDK-tagged fusion protein (25 ng).
Lane 2 : C-terminal DYKDDDDK-tagged fusion protein (10 ng).
Lane 3 : C-terminal DYKDDDDK-tagged fusion protein (5 ng).
Lane 4 : C-terminal DYKDDDDK-tagged fusion protein (1 ng).
Primary Antibody: Anti-Flag Tag Antibody-HRP (3S989) (TMAS-00123H) 1 μg/ml.
3. Sensitivity and specificity of Anti-Flag Tag Antibody-HRP (3S989) (TMAS-00123H) to N-DYKDDDDK-tag Fusion Protein by Western blot.
Lane 1 : N-terminal DYKDDDDK-tagged fusion protein (25 ng).
Lane 2 : N-terminal DYKDDDDK-tagged fusion protein (10 ng).
Lane 3 : N-terminal DYKDDDDK-tagged fusion protein (5 ng).
Lane 4 : N-terminal DYKDDDDK-tagged fusion protein (1 ng).
Primary Antibody: Anti-Flag Tag Antibody-HRP (3S989) (TMAS-00123H) 1 μg/ml.
Application
ELISA
WB
Recommended Dose
ELISA: 0.05-0.2 μg/ml; WB: 0.5-1 μg/ml
Antibody Type
Monoclonal
Host SpeciesRabbit
PurificationProtein A affinity column
AppearanceLyophilized. Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
FormulationLyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal.
Research Background0
Related Conjugates and Formulations
Conjucates
HRP
Others Formats
Unconjugated/Biotin/FITC
Antibody Types Available
4
Antigen Details
Immunogen
KLH conjugated synthetic peptide: DYKDDDDK
Chemical Properties
Stability & Storage
Stability & StorageThe lyophilized product store at -20°C or -80°C for 12 months. The reconstituted antibody can be stored at 2°C-8°C for 2-3 weeks or at -20°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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