HER2/ERBB2 Protein, Human, Recombinant (aa 1-652, His)

Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Pack Size	Availability	Price/USD
100 μg	In stock	$ 329.00
200 μg	5 days	$ 583.00
500 μg	5 days	$ 1,230.00

Pack Size

Availability

Price/USD

Quantity

100 μg

In stock

$ 329.00

200 μg

5 days

$ 583.00

500 μg

5 days

$ 1,230.00

Biological Information

1. Immobilized Her2/ERBB2 Protein, Human, Recombinant (ECD, His Tag) at 2 μg/ml (100 μl/well) can bind Anti-Erbb2 Antibody (Trastuzumab), the EC50 is 10-60 ng/mL. 2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.2-1 μg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody. 3. Using the Octet RED System, the affinity constant (Kd) of Her2 / ERBB2 Protein, Human, Recombinant (His Tag) bound to Pertuzumab was 0.1 nM. 4.Captured anti-Her2 IgG1 antibody on proA Chip can bind Her2 with an affinity constant of 3.270 nM as determined in a SPR assay (Routinely tested). 5.Captured Trastuzumab on proA Chip can bind Her2 with an affinity constant of 3.727 nM as determined in a SPR assay(Routinely tested).

Description

Species	Human
Expression System	HEK293
Tag	His
Accession Number	P04626-1
Synonyms	erb-b2 receptor tyrosine kinase 2, NEU, ENVW, HERV-7q, HERV7Q, HER-2/neu, MLN 19, ENV, TKR1, EGFR2, HERV-W-ENV, MLN19, HERVWENV, ERVWE1, HER-2, CD340, NGL, HER2, HERVW
Construction	A DNA sequence encoding the extracellular domain (Met 1-Thr 652) of human ErbB2 (NP_004439.2) was fused with the polyhistidine tag at the C-terminus.
Protein Purity	> 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
Molecular Weight	Approxiamtely 71 kDa

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy