CD30 Ligand/TNFSF8 Protein, Human, Recombinant (His)

Catalog No. TMPK-00122

CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.

CD30 Ligand/TNFSF8 Protein, Human, Recombinant (His)

Catalog No. TMPK-00122

Pack Size	Price	Availability
5 μg	$58	7-10 days
10 μg	$92	7-10 days
20 μg	$148	7-10 days
50 μg	$289	7-10 days
100 μg	$487	7-10 days
200 μg	$883	7-10 days
500 μg	$1,950	7-10 days

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Product Information

Biological Activity	Immobilized Human CD30 Ligand, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 8.9ng/ml determined by ELISA.
Description	CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
Species	Human
Expression System	HEK293 Cells
Tag	N-His
Accession Number	P32971
Synonyms	TNFSF8,CD30LGMGC138144,CD30LG,CD30-L,CD30L,CD30 Ligand,CD153
Construction	Gln63-Asp234
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	20.7 kDa (predicted). Due to glycosylation, the protein migrates to 30-50 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.