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SARS-CoV-2 Spike S1 ECD Protein (D614G, hFc)

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Catalog No. TMPY-06008 Copy Product Info
SARS-CoV-2 Spike S1 ECD Protein (D614G, hFc) is expressed in HEK293 mammalian cells. The predicted molecular weight is 101.7 kDa and the accession number is A0A6G7K2L4.
SARS-CoV-2 Spike S1 ECD Protein (D614G, hFc)
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Pack SizePriceUSA StockGlobal StockQuantity
5 μg$777-10 days7-10 days
10 μg$1237-10 days7-10 days
20 μg$1987-10 days7-10 days
50 μg$3977-10 days7-10 days
100 μg$7937-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1(D614G), ECD-Fc, the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1(D614G),ECD-Fc is 40-230 ng/mL.
Description
SARS-CoV-2 Spike S1 ECD Protein (D614G, hFc) is expressed in HEK293 mammalian cells. The predicted molecular weight is 101.7 kDa and the accession number is A0A6G7K2L4.
Species
SARS-CoV-2
Expression System
HEK293 Cells
TagC-hFc
Accession NumberA0A6G7K2L4
ConstructionA DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-Fc Recombinant Protein (YP_009724390.1) (Val16-Arg685(D614G)) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Val 16
Protein Purity
> 90 % as determined by SDS-PAGE.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background
Chemical Properties
Molecular Weight101.7 kDa (predicted)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: SARS-CoV-2 Spike S1 ECD Protein (D614G, hFc) chemical structure | SARS-CoV-2 Spike S1 ECD Protein (D614G, hFc) molecular weight