PCSK9 Protein, Human, Recombinant (D374Y & V474I & G670E, His)

Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.

Pack Size	Availability	Price/USD
50 μg	In stock	$ 386.00
100 μg	5 days	$ 602.00
200 μg	5 days	$ 939.00
500 μg	5 days	$ 1,690.00

Pack Size

Availability

Price/USD

Quantity

50 μg

In stock

$ 386.00

100 μg

5 days

$ 602.00

200 μg

5 days

$ 939.00

500 μg

5 days

$ 1,690.00

Biological Information

1.Measured by its binding ability in a functional ELISA. Immobilized human PCSK9-His(D374Y)at 10 μg/mL (100 μL /well) can bind biotinylated LDLR-His , The EC50 of biotinylated LDLR-His is 0.2-1.4μg/mL. 2.Loaded Recombinant Human LDLR/LDL Receptor Protein, mFc Tag on ProA Biosensor, can bind Human PCSK9 / NARC1 (D374Y, V474I, G670E) Protein, His Tag with an affinity constant of 3.08nM as determined in BLI assay (Routinely tested).

Description

Species	Human
Expression System	HEK293
Tag	His
Accession Number	AAV67948.1
Synonyms	FH3, proprotein convertase subtilisin/kexin type 9, LDLCQ1, HCHOLA3, NARC1, PC9, NARC-1
Construction	A DNA sequence encoding the human PCSK9 (NP_777596.2, with mutation Asp 374 Tyr, natural mutation Val 474 Ile and Gly 670 Glu) (Met 1-Gln 692) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Molecular Weight	Approxiamtely 72.6 kDa

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile 20mM Tris, 100mM NaCl, 10% Glycerol, 0. 01% Tween20, pH 8.5. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.