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Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9S543)

🥰Excellent
Catalog No. TMAY-02875

Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9S543) is a Rabbit antibody targeting SARS-CoV/SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9S543) can be used in WB,ELISA,IHC-P,ICC/IF.

Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9S543)

Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9S543)

🥰Excellent
Catalog No. TMAY-02875
Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9S543) is a Rabbit antibody targeting SARS-CoV/SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9S543) can be used in WB,ELISA,IHC-P,ICC/IF.
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Product Introduction

Bioactivity
Description
Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9S543) is a Rabbit antibody targeting SARS-CoV/SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9S543) can be used in WB,ELISA,IHC-P,ICC/IF.
Ig Type
Monoclonal Rabbit IgG
Clone
9S543
Verified Activity
1. Anti-SARS-NP rabbit monoclonal antibody at 1:2000 dilution.

-Lane A: SARS-CoV NP Protein (30ng)

-Lane B: SARS-CoV NP Protein (5ng)

-Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (30ng)

-Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (5ng)

-Secondary

-Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

-Developed using the ECL technique.

-Performed under reducing conditions.

2. Immunochemical analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS Nucleocapsid Rabbit Mab,then a HRP-conjugated second step antibody.

3. Immunofluorescence analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS Nucleocapsid Rabbit Mab,then a Alexa Fluor®488-conjugated second step antibody. (Validation Experiment)
Application
WB,ELISA,IHC-P,ICC/IF
Recommended Dose
WB: 1:5000-1:20000; ELISA: 1:5000-1:10000; IHC-P: 1:5000-1:20000; ICC-IF: 1:20-1:100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis product is a recombinant monoclonal antibody expressed from HEK293 cells.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundCoronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
Biotin/HRP
Antibody Types Available
3
Antigen Details
Immunogen
Recombinant SARS-CoV Nucleoprotein / NP Protein (TMPY-02870)
Antigen Species
SARS
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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