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Anti-Phospho-PRKAA2 (Thr172) Antibody (7F577)
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Catalog No. TMAH-00945
Alias Protein kinase AMP activated catalytic subunit alpha 2, Protein kinase AMP activated alpha 2 catalytic subunit, PRKAA 2, PRKAA, Phospho-PRKAA2 (T172), Hydroxymethylglutaryl CoA reductase kinase, HMGCR kinase, AMPKalpha2, AMPKa2, AMPK2, AMPK subunit alpha-2, AMPK alpha 2 chain, Acetyl-CoA carboxylase kinase, ACACA kinase, AAPK2_HUMAN, 5'-AMP-activated protein kinase catalytic subunit alpha-2
Anti-Phospho-PRKAA2 (Thr172) Antibody (7F577) is an antibody targeting Phospho-PRKAA2 (Thr172). Anti-Phospho-PRKAA2 (Thr172) Antibody (7F577) can be used in ELISA, WB, IF.
Anti-Phospho-PRKAA2 (Thr172) Antibody (7F577)
Anti-Phospho-PRKAA2 (Thr172) Antibody (7F577)
😃Good
Catalog No. TMAH-00945Alias Protein kinase AMP activated catalytic subunit alpha 2, Protein kinase AMP activated alpha 2 catalytic subunit, PRKAA 2, PRKAA, Phospho-PRKAA2 (T172), Hydroxymethylglutaryl CoA reductase kinase, HMGCR kinase, AMPKalpha2, AMPKa2, AMPK2, AMPK subunit alpha-2, AMPK alpha 2 chain, Acetyl-CoA carboxylase kinase, ACACA kinase, AAPK2_HUMAN, 5'-AMP-activated protein kinase catalytic subunit alpha-2
Anti-Phospho-PRKAA2 (Thr172) Antibody (7F577) is an antibody targeting Phospho-PRKAA2 (Thr172). Anti-Phospho-PRKAA2 (Thr172) Antibody (7F577) can be used in ELISA, WB, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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| Description | Anti-Phospho-PRKAA2 (Thr172) Antibody (7F577) is an antibody targeting Phospho-PRKAA2 (Thr172). Anti-Phospho-PRKAA2 (Thr172) Antibody (7F577) can be used in ELISA, WB, IF. |
| Alias | Protein kinase AMP activated catalytic subunit alpha 2, Protein kinase AMP activated alpha 2 catalytic subunit, PRKAA 2, PRKAA, Phospho-PRKAA2 (T172), Hydroxymethylglutaryl CoA reductase kinase, HMGCR kinase, AMPKalpha2, AMPKa2, AMPK2, AMPK subunit alpha-2, AMPK alpha 2 chain, Acetyl-CoA carboxylase kinase, ACACA kinase, AAPK2_HUMAN, 5'-AMP-activated protein kinase catalytic subunit alpha-2 |
| Ig Type | Rabbit IgG |
| Clone | 7F577 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in 293 whole cell lysate, A549 whole cell lysate(treated with Calyculin A or EGF) -All lanes Phospho-PRKAA2 antibody at 1.95μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 62 KDa -Observed band size: 62 KDa 2. Immunofluorescence staining of A549 cells with TMAH-00945 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | ELISA, WB, IF |
| Recommended Dose | WB:1:500-1:5000; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus. Note=In response to stress, recruited by p53/TP53 to specific promoters. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the a... |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Phospho-PRKAA2 (Thr172) |
| Antigen Species | Human |
| Gene ID | 5563 |
| Uniprot ID | |
| Biology Area | Signal Transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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