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Anti-Phospho-PRKAA2 (Thr172) Antibody (7F577)
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Catalog No. TMAH-00945
Alias Protein kinase AMP activated catalytic subunit alpha 2, Protein kinase AMP activated alpha 2 catalytic subunit, PRKAA2 (p-Thr172), PRKAA2 (p-T172), PRKAA 2, PRKAA, p-PRKAA2 (Thr172), p-PRKAA2 (T172), Phospho-PRKAA2 (T172), Hydroxymethylglutaryl CoA reductase kinase, HMGCR kinase, AMPKalpha2, AMPKa2, AMPK2, AMPK subunit alpha-2, AMPK alpha 2 chain, Acetyl-CoA carboxylase kinase, ACACA kinase, AAPK2_HUMAN, 5'-AMP-activated protein kinase catalytic subunit alpha-2
Anti-Phospho-PRKAA2 (Thr172) Antibody (7F577) is an antibody targeting Phospho-PRKAA2 (Thr172). Anti-Phospho-PRKAA2 (Thr172) Antibody (7F577) can be used in ELISA, WB, IF.
Anti-Phospho-PRKAA2 (Thr172) Antibody (7F577)
Anti-Phospho-PRKAA2 (Thr172) Antibody (7F577)
😃Good
Catalog No. TMAH-00945Alias Protein kinase AMP activated catalytic subunit alpha 2, Protein kinase AMP activated alpha 2 catalytic subunit, PRKAA2 (p-Thr172), PRKAA2 (p-T172), PRKAA 2, PRKAA, p-PRKAA2 (Thr172), p-PRKAA2 (T172), Phospho-PRKAA2 (T172), Hydroxymethylglutaryl CoA reductase kinase, HMGCR kinase, AMPKalpha2, AMPKa2, AMPK2, AMPK subunit alpha-2, AMPK alpha 2 chain, Acetyl-CoA carboxylase kinase, ACACA kinase, AAPK2_HUMAN, 5'-AMP-activated protein kinase catalytic subunit alpha-2
Anti-Phospho-PRKAA2 (Thr172) Antibody (7F577) is an antibody targeting Phospho-PRKAA2 (Thr172). Anti-Phospho-PRKAA2 (Thr172) Antibody (7F577) can be used in ELISA, WB, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IF Reactivity: Human |
| Alias | Protein kinase AMP activated catalytic subunit alpha 2, Protein kinase AMP activated alpha 2 catalytic subunit, PRKAA2 (p-Thr172), PRKAA2 (p-T172), PRKAA 2, PRKAA, p-PRKAA2 (Thr172), p-PRKAA2 (T172), Phospho-PRKAA2 (T172), Hydroxymethylglutaryl CoA reductase kinase, HMGCR kinase, AMPKalpha2, AMPKa2, AMPK2, AMPK subunit alpha-2, AMPK alpha 2 chain, Acetyl-CoA carboxylase kinase, ACACA kinase, AAPK2_HUMAN, 5'-AMP-activated protein kinase catalytic subunit alpha-2 |
| Ig Type | Rabbit IgG |
| Clone | 7F577 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in 293 whole cell lysate, A549 whole cell lysate(treated with Calyculin A or EGF) -All lanes Phospho-PRKAA2 antibody at 1.95μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 62 KDa -Observed band size: 62 KDa 2. Immunofluorescence staining of A549 cells with TMAH-00945 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | ELISA, WB, IF |
| Recommended Dose | WB:1:500-1:5000; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus. Note=In response to stress, recruited by p53/TP53 to specific promoters. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. Involved in insulin receptor/INSR internalization. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. In that process also activates WDR45. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1. Plays an important role in the differential regulation of pro-autophagy (composed of PIK3C3, BECN1, PIK3R4 and UVRAG or ATG14) and non-autophagy (composed of PIK3C3, BECN1 and PIK3R4) complexes, in response to glucose starvation. Can inhibit the non-autophagy complex by phosphorylating PIK3C3 and can activate the pro-autophagy complex by phosphorylating BECN1. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Phospho-PRKAA2 (Thr172) |
| Antigen Species | Human |
| Gene ID | 5563 |
| Uniprot ID | |
| Biology Area | Signal Transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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