MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type II receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without the capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+αβ T cells, and γδ T cells on which the receptor is expressed. MICA/B is minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | In stock | $ 386.00 | |
200 μg | 5 days | $ 682.00 | |
500 μg | 5 days | $ 1,440.00 |
Biological Information |
Immobilized human MICB-His at 10 μg/ml (100 μl/well) can bind S4-Fc3L3-NKG2D , The EC50 of S4-Fc3L3-NKG2D is 0.52-1.2 μg/ml.
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Description | MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type II receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without the capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+αβ T cells, and γδ T cells on which the receptor is expressed. MICA/B is minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases. |
Species | Human |
Expression System | HEK293 |
Tag | His |
Accession Number | A0A7D9H7X8 |
Synonyms | MHC class I polypeptide-related sequence B, PERB11.2 |
Construction | A DNA sequence encoding the extracellular domain of human MICB (NP_005922.2) (Met 1-Gly 298) was expressed with a C-terminal polyhistidine tag. |
Protein Purity |
≥ 98 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
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Molecular Weight | Approxiamtely 33 kDa |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Reconstitution | A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type II receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without the capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+αβ T cells, and γδ T cells on which the receptor is expressed. MICA/B is minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases. |
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Please read the User Guide of Recombinant Proteins for more specific information.
MICB Protein, Human, Recombinant (His) MHC class I polypeptide-related sequence B PERB11.2 recombinant recombinant-proteins proteins protein