Fas/CD95 Protein, Rat, Recombinant (hFc)

Name: Fas/CD95 Protein, Rat, Recombinant (hFc)
Brand: TargetMol
SKU: TMPY-04282
Rating: 4.5 (1 reviews)

(Synonyms: Fas cell surface death receptor) Copy Product Info

Synonyms: Fas cell surface death receptor

Catalog No. TMPY-04282 Copy Product Info

Fas/CD95 Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 43.7 kDa and the accession number is A6I127.

Pack Size	Price	EUR Stock	Global Stock
5 µg	61 €	7-10 days	7-10 days
10 µg	97 €	7-10 days	7-10 days
20 µg	160 €	7-10 days	7-10 days
50 µg	323 €	7-10 days	7-10 days
100 µg	626 €	7-10 days	7-10 days

For In stock only · Estimated delivery: EUR Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized human His-FASLG at 10 μg/ml (100 μl/well) can bind rat FAS-Fc. The EC50 of rat FAS-Fc is 0.22-0.52 μg/ml.
Description	Fas/CD95 Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 43.7 kDa and the accession number is A6I127.
Species	Rat
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	A6I127
Construction	A DNA sequence encoding the rat FAS (NP_631933.2) (Met1-Lys170) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Gln 22
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	Fas cell surface death receptor
Research Background	CD95 (APO-1/Fas) is an important inducer of the extrinsic apoptosis signaling pathway and therapy induced apoptosis of many tumor cells has been linked to the activity of CD95. is a prototype death receptor characterized by the presence of an 80 amino acid death domain in its cytoplasmic tail. This domain is essential for the recruitment of a number of signaling components upon activation by either agonistic anti-CD95 antibodies or cognate CD95 ligand that initiate apoptosis. The complex of proteins that forms upon triggering of CD95 is called the death-inducting signaling complex (DISC). The DISC consists of an adaptor protein and initiator caspases and is essential for induction of apoptosis. CD95 is also crucial for the negative selection of B cells within the germinal center (GC). Impairment of CD95-mediated apoptosis results in defective affinity maturation and the persistence of autoreactive B-cell clones. Changes in the expression of CD95 and/or its ligand CD95L are frequently found in human cancer. The downregulation or mutation of CD95 has been proposed as a mechanism by which cancer cells avoid destruction by the immune system through reduced apoptosis sensitivity. Thus, CD95 has therefore been viewed as a tumor suppressor. CD95 has been reported to be involved in the activation of NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and the alternate pathways for CTL-mediated cytotoxicity. Accordingly, this protein is implicated in the pathogenesis of various malignancies and diseases of the immune system. The CD95/CD95L system was implicated in the etiology of inflammatory bowel disease (IBD) based, primarily, on the finding that CD95 is highly expressed in the intestinal epithelial cells and that epithelial apoptosis is increased in IBD.

Chemical Properties

Molecular Weight

43.7 kDa (predicted); 57 and 36 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.