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Anti-KRT6A Antibody (4O729)
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Catalog No. TMAH-00678
Alias Type-II keratin Kb6, type II cytoskeletal 6A, KRT 6A, Keratin-6A, Keratin, K6A KRT6D, K6A, Cytokeratin-6D, Cytokeratin-6A, CK-6D, CK-6A
Anti-KRT6A Antibody (4O729) is an antibody targeting KRT6A. Anti-KRT6A Antibody (4O729) can be used in ELISA, IF, FCM.
Anti-KRT6A Antibody (4O729)
Anti-KRT6A Antibody (4O729)
😃Good
Catalog No. TMAH-00678Alias Type-II keratin Kb6, type II cytoskeletal 6A, KRT 6A, Keratin-6A, Keratin, K6A KRT6D, K6A, Cytokeratin-6D, Cytokeratin-6A, CK-6D, CK-6A
Anti-KRT6A Antibody (4O729) is an antibody targeting KRT6A. Anti-KRT6A Antibody (4O729) can be used in ELISA, IF, FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, IF, FCM Reactivity: Human |
| Alias | Type-II keratin Kb6, type II cytoskeletal 6A, KRT 6A, Keratin-6A, Keratin, K6A KRT6D, K6A, Cytokeratin-6D, Cytokeratin-6A, CK-6D, CK-6A |
| Ig Type | Rabbit IgG |
| Clone | 4O729 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of A549 with TMAH-00678 at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 519-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). 2. Overlay Peak curve showing Hela cells stained with TMAH-00678 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, IF, FCM |
| Recommended Dose | IF:1:50-1:200; FCM:1:50-1:200. |
| Antibody Type | Monoclonal |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human KRT6A |
| Antigen Species | Human |
| Gene ID | 3853 |
| Uniprot ID | |
| Biology Area | Tags & Cell Markers, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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