Antibody Type: Recombinant Monoclonal

Application: ELISA, WB, IHC, FC

Reactivity: Human

hIgG1

7R530

Human

1. Western Blot
-Positive WB detected in:K562 whole cell lysate (20µg), A375 whole cell lysate (20µg)
-All lanes: ICAM1 antibody at 1:1000
-Secondary: Goat polyclonal to human IgG at 1/40000 dilution
-Predicted band size:57.83 kDa
-Observed band size:58 kDa
-Exposure time:120s
2. IHC image of TMAH-00589 diluted at 1：200 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
3. Overlay Peak curve showing HepG2 cells stained with TMAH-00589 (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was human IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IHC, FC

WB:1:500-1:2000; IHC:1:50-1:200; FC:1:50-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human ICAM1 Protein

Human

3383

Cardiovascular, Immunology, Stem cells