Antibody Type: Mouse Monoclonal

Application: ELISA, WB, IP

Reactivity: All

IgG1

4Q485

All

1. Western Blot
-Positive WB detected in: 200ng recombinant protein with His tag His antibody at 1:8w, 1:16w, 1:32w, 1:64w, 1:128w, 1:256w
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 43.4 KDa
-Observed band size: 43.4 KDa
-Exposure time：5min
2. Western Blot
-Positive WB detected in: 200ng recombinant protein with His tag His antibody at 1:2w, 1:4w, 1:8w, 1:16w, 1:32w, 1:64w
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size:62.5 KDa
-Observed band size: 66 KDa
-Exposure time：5min
3. Western Blot
-Positive WB detected in:200ng of four Recombinant protein with his tag -All lanes: His antibody at 1:5000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 43.4, 62.5,56,80.6 KDa
-Observed band size:43.4, 66, 57,90 KDa
-Exposure time：5min
4. Immunoprecipitating His in 293 whole cell lysate transfected with His
-Lane 1: control IgG instead of 2F4B5 in 293 whole cell lysate transfected with His. -Lane 2: 2F4B5 antibody (2µg) + 293 whole cell lysate transfected with His (500µg)
-Lane 3: 293 whole cell lysate transfected with His (5µg)
For western blotting, the blot was detected with 2F4B5 antibody at 1:10000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000

ELISA, WB, IP

WB:1:1000-1:640000; IP:2ul-5ul.

Monoclonal

Unconjugated

The immunogen is a mixture of various His-tagged Proteins

Others