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Anti-His Antibody (4Q485) (Synonyms: His Tag, HHHHHH, 6histag, 6*His)
Catalog No. TMAH-00545 Copy Product Info
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Anti-His Antibody (4Q485) is a Mouse antibody targeting His. Anti-His Antibody (4Q485) can be used in ELISA,IP,WB.
Anti-His Antibody (4Q485)
Copy Product Info🥰Excellent
Catalog No. TMAH-00545
Synonyms His Tag, HHHHHH, 6histag, 6*His
Anti-His Antibody (4Q485) is a Mouse antibody targeting His. Anti-His Antibody (4Q485) can be used in ELISA,IP,WB.
Anti-His Antibody
(4Q485)
(4Q485)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $119 | 7-10 days | 7-10 days | |
| 100 μL | $197 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:>95%, Protein G purified
Appearance:Liquid
Color:Transparent
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Product Introduction
Bioactivity
Related Conjugates and Formulations
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Chemical Properties
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| Description | Anti-His Antibody (4Q485) is a Mouse antibody targeting His. Anti-His Antibody (4Q485) can be used in ELISA,IP,WB. |
| Synonyms | His Tag, HHHHHH, 6histag, 6*His |
| Ig Type | IgG1 |
| Clone | 4Q485 |
| Reactivity | All |
| Verified Activity | 1. Western Blot -Positive WB detected in: 200ng recombinant protein with His tag His antibody at 1:8w, 1:16w, 1:32w, 1:64w, 1:128w, 1:256w -Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution -Predicted band size: 43.4 KDa -Observed band size: 43.4 KDa -Exposure time：5min 2. Western Blot -Positive WB detected in: 200ng recombinant protein with His tag His antibody at 1:2w, 1:4w, 1:8w, 1:16w, 1:32w, 1:64w -Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution -Predicted band size:62.5 KDa -Observed band size: 66 KDa -Exposure time：5min 3. Western Blot -Positive WB detected in:200ng of four Recombinant protein with his tag -All lanes: His antibody at 1:5000 -Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution -Predicted band size: 43.4, 62.5,56,80.6 KDa -Observed band size:43.4, 66, 57,90 KDa -Exposure time：5min 4. Immunoprecipitating His in 293 whole cell lysate transfected with His -Lane 1: control IgG instead of 2F4B5 in 293 whole cell lysate transfected with His. -Lane 2: 2F4B5 antibody (2µg) + 293 whole cell lysate transfected with His (500µg) -Lane 3: 293 whole cell lysate transfected with His (5µg) For western blotting, the blot was detected with 2F4B5 antibody at 1:10000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000 |
| Application | |
| Recommended Dose | WB:1:1000-1:640000; IP:2ul-5ul. |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Purity | >95% |
| Conjucates | Unconjugated |
| Immunogen | The immunogen is a mixture of various His-tagged Proteins |
| Biology Area | Others |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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