PCSK9 Protein, Human, Recombinant (D374Y, His)

Catalog No. TMPY-04966 Copy Product Info

PCSK9 Protein, Human, Recombinant (D374Y, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 72.5 kDa and the accession number is Q8NBP7-1.

PCSK9 Protein, Human, Recombinant (D374Y, His)

Catalog No. TMPY-04966

Copy Product Info

PCSK9 Protein, Human, Recombinant (D374Y, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 72.5 kDa and the accession number is Q8NBP7-1.

Pack Size	Price	USA Stock	Global Stock
5 μg	$75	7-10 days	7-10 days
10 μg	$118	7-10 days	7-10 days
20 μg	$196	7-10 days	7-10 days
50 μg	$386	-	In Stock
100 μg	$602	7-10 days	7-10 days
200 μg	$939	7-10 days	7-10 days
500 μg	$1,690	7-10 days	7-10 days

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For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)

For research use only—not for human use. No sales to individuals. Use as intended only.

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Biological Activity	1.Measured by its binding ability in a functional ELISA. Immobilized Human LDLR mFc at 2 μg/ml (100 μl/well) can bind Human PCSK9(D374Y) His, the EC50 of PCSK9（D374Y) His is 1-20 ng/mL. 2.Loaded Recombinant Human LDLR/LDL Receptor Protein, mFc Tag on ProA Biosensor, can bind Recombinant Human PCSK9 (D374Y) Protein, His Tag with an affinity constant of 2.57nM as determined in BLI assay (Routinely tested).
Description	PCSK9 Protein, Human, Recombinant (D374Y, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 72.5 kDa and the accession number is Q8NBP7-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q8NBP7-1
Synonyms	proprotein convertase subtilisin/kexin type 9,PC9,NARC-1,NARC1,LDLCQ1,HCHOLA3,FH3
Construction	A DNA sequence encoding the human PCSK9 (NP_777596.2, with mutation Asp 374 Tyr) (Met1-Gln692) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Gln 31
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Molecular Weight	72.5 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background	Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.