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Anti-Flagellin Antibody (3P374)

😃Good
Catalog No. TMAY-02860
Alias flaA

Anti-Flagellin Antibody (3P374) is a Rabbit antibody targeting Flagellin. Anti-Flagellin Antibody (3P374) can be used in ELISA.

Anti-Flagellin Antibody (3P374)

Anti-Flagellin Antibody (3P374)

😃Good
Catalog No. TMAY-02860Alias flaA
Anti-Flagellin Antibody (3P374) is a Rabbit antibody targeting Flagellin. Anti-Flagellin Antibody (3P374) can be used in ELISA.
Pack SizePriceAvailabilityQuantity
20 μL$92 7-10 days
100 μL$270 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Monoclonal

Application: ELISA

Reactivity: Listeria monocytogenes
AliasflaA
Ig Type
Rabbit IgG
Clone
3P374
Reactivity
Listeria monocytogenes
Application
ELISA
Recommended Dose
ELISA: 1:5000-1:10000
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Listeria monocytogenes Listeria monocytogenes flagellin / flaA (TMPY-03465; YP_013330.1; Met1-Ser287).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundThe role of flagella and motility in the attachment of the foodborne pathogen Listeria monocytogenes to various surfaces is mixed with some systems requiring flagella for an interaction and others needing only motility for cells to get to the surface. In nature this bacterium is a saprophyte and contaminated produce is an avenue for infection. Previous studies have documented the ability of this organism to attach to and colonize plant tissue. Motility mutants were generated in three wild type strains of L. monocytogenes by deleting either FlaA, the gene encoding flagellin, or motAB, genes encoding part of the flagellar motor, and tested for both the ability to colonize sprouts and for the fitness of that colonization. The motAB mutants were not affected in the colonization of alfalfa, radish, and broccoli sprouts; however, some of the FlaA mutants showed reduced colonization ability. The best colonizing wild type strain was reduced in colonization on all three sprout types as a result of a FlaA deletion. A mutant in another background was only affected on alfalfa. The third, a poor alfalfa colonizer was not affected in colonization ability by any of the deletions. Fitness of colonization was measured in experiments of competition between mixtures of mutant and parent strains on sprouts. Here the FlaA and motAB mutants of the three strain backgrounds were impaired in fitness of colonization of alfalfa and radish sprouts, and one strain background showed reduced fitness of both mutant types on broccoli sprouts. Together these data indicate a role for flagella for some strains to physically colonize some plants, while the fitness of that colonization is positively affected by motility in almost all cases.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Listeria monocytogenes Listeria monocytogenes flagellin / FlaA protein (TMPY-03465)
Antigen Species
Listeria monocytogenes
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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