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Anti-Phospho-STAT5A (Tyr394) Antibody (3K823) is an antibody targeting Phospho-STAT5A (Tyr394). Anti-Phospho-STAT5A (Tyr394) Antibody (3K823) can be used in ELISA, WB, IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $209 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC Reactivity: Human |
Alias | Transcription factor STAT5B, Transcription factor STAT5A, STAT5B, STAT5A (p-Y694), STAT5A (p-Tyr394), STAT5, STAT 5B, STAT 5A, STA5A_HUMAN, Signal Transducer and Activator of Transcription 5B, Signal transducer and activator of transcription 5A, p-STAT5A (Y694), p-STAT5A (Tyr394), Phospho-STAT5A (Y694), MGF |
Ig Type | Rabbit IgG |
Clone | 3K823 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in HepG2 whole cell lysate(treated with EGF or not) -All lanes Phospho-STAT5A antibody at 1.025μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 90 KDa -Observed band size: 90 KDa 2. IHC image of TMAH-00972 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Application | ELISA, WB, IHC |
Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Carries out a dual signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human Phospho-STAT5A (Y694) |
Antigen Species | Human |
Gene ID | 6776 |
Uniprot ID | |
Biology Area | Signal Transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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