1. Immobilized IL-5 Protein, Human, Recombinant (mFc) (Cat#TMPY-05536) at 2 μg/mL (100 μL/well) can bind IL-5R alpha/CD125 Protein, Human, Recombinant (Membrane-bound, His) (Cat#TMPY-07016), the EC50 is 60-220 ng/mL.
2. Loaded IL-5R alpha/CD125 Protein, Human, Recombinant (Membrane-bound, His) (Cat#TMPY-07016) on HIS1K Biosensor, can bind IL-5 Protein, Human, Recombinant (HEK293) (Cat#TMPY-07129) with an affinity constant of 43.200 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
3. Loaded Biotinylated Recombinant Human IL-5 Protein, His & AVI Tag on SA Biosensor, can bind IL-5R alpha/CD125 Protein, Human, Recombinant (Membrane-bound, His) (Cat#TMPY-07016) with an affinity constant of 17.800 nM as determined in BLI assay (Sartorius Octet RED384) (QC tested).

IL-5R alpha/CD125 Protein, Human, Recombinant (Membrane-bound, His) is expressed in HEK293 Cells. The accession number is Q01344-1.

Human

HEK293 Cells

IL5R,HSIL5R3,CDw125,CD125

A DNA sequence encoding the Human IL5RA (Q01344-1) (Met1-Trp342) was expressed with a polyhistidine tag at the C-terminus.

≥ 95% as determined by SDS-PAGE. ≥ 95% as determined by SEC-HPLC.

Please refer to the lot-specific COA.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Interleukin 5 receptor, alpha (IL5RA) also known as CD125 (Cluster of Differentiation 125) is a subunit of the Interleukin-5 receptor. IL5RA (CD125) is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand-specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony-stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Six alternatively spliced transcript variants encoding three distinct isoforms have been reported. IL5RA (CD125) is a T-cell-derived cytokine that is particularly important in the development of asthma for the terminal differentiation, activation, and survival of committed eosinophil precursors.