Ephrin B3/EFNB3 Protein, Rat, Recombinant (His)

Name: Ephrin B3/EFNB3 Protein, Rat, Recombinant (His)
Brand: TargetMol
SKU: TMPY-03114
Rating: 4.5 (1 reviews)

(Synonyms: ephrin-B3) Copy Product Info

Synonyms: ephrin-B3

Catalog No. TMPY-03114 Copy Product Info

Ephrin B3/EFNB3 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.1 kDa and the accession number is G3V7D4.

Pack Size	Price	USA Stock	Global Stock
5 µg	$55	7-10 days	7-10 days
10 µg	$87	7-10 days	7-10 days
20 µg	$142	7-10 days	7-10 days
50 µg	$277	7-10 days	7-10 days
100 µg	$535	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized rat EFNB3-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse EphB3-His , The EC50 of biotinylated mouse EphB3-His is 17.4-40.6 ng/ml.
Description	Ephrin B3/EFNB3 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.1 kDa and the accession number is G3V7D4.
Species	Rat
Expression System	HEK293 Cells
Tag	C-His
Accession Number	G3V7D4
Construction	A DNA sequence encoding the rat EFNB3 (G3V7D4) (Met1-Ser224) was expressed, fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Leu 28
Protein Purity	> 85 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	ephrin-B3
Research Background	Ephrin B3 belongs to the ephrin family. Ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin B3 is important in brain development as well as in its maintenance. It is especially important for forebrain function since its expression levels were particularly high in several forebrain subregions compared to other brain subregions. Ephrin B3 binds to, and induce the collapse of, commissural axons/growth cones in vitro. It may play a role in constraining the orientation of longitudinally projecting axons.

Chemical Properties

Molecular Weight

23.1 kDa (predicted); 32 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.