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Anti-Cathepsin D Antibody (5Z751)
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Catalog No. TMAY-03121
Alias HEL-S-130P, CPSD, CLN10, cathepsin D
Anti-Cathepsin D Antibody (5Z751) is a Rabbit antibody targeting Cathepsin D. Anti-Cathepsin D Antibody (5Z751) can be used in WB,ELISA,IP.
Anti-Cathepsin D Antibody (5Z751)
Anti-Cathepsin D Antibody (5Z751)
😃Good
Catalog No. TMAY-03121Alias HEL-S-130P, CPSD, CLN10, cathepsin D
Anti-Cathepsin D Antibody (5Z751) is a Rabbit antibody targeting Cathepsin D. Anti-Cathepsin D Antibody (5Z751) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Cathepsin D Antibody (5Z751) is a Rabbit antibody targeting Cathepsin D. Anti-Cathepsin D Antibody (5Z751) can be used in WB,ELISA,IP. |
| Alias | HEL-S-130P, CPSD, CLN10, cathepsin D |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 5Z751 |
| Reactivity | Mouse |
| Verified Activity | 1. Mouse CTSD was immunoprecipitated using: -Lane A:0.5 mg MCF-7 Whole Cell Lysate. -Lane B:0.5 mg HepG2 Whole Cell Lysate -0.5 µL anti-Mouse CTSD rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-Mouse CTSD rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 45 kDa. -Observed band size: 45 kDa. 2. Anti-CTSD rabbit monoclonal antibody at 1:500 dilution. -Lane A: MCF7 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:45 kDa. -Observed band size:45 kDa(We are unsure as to the identity of these extra bands.) |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:1000; ELISA: 1:25000-1:50000; IP: 1-2 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Cathepsin D / CTSD (rM Cathepsin D / CTSD; TMPY-00733; NP_034113.1; Met 1-Leu 410). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Cathepsin D (CTSD), a well known lysosomal aspartyl protease and belongs to the peptidase C1 family, which is a normal and major component of lysosomes, and is found in almost all cells and tissues of mammals. Its mostly described function is intracellular catabolism in lysosomal compartments, other physiological effect include hormone and antigen processing. Cathepsin D has a specificity similar to but narrower than that of pepsin A. Cathepsin D plays an important role in the degradation of proteins, the generation of bioactive proteins, antigen processing, etc. Among different role in cell physiology, a new function of this enzyme is examined. Cathepsin D is an important regulator of apoptotic pathways in cells. It acts at different stage of intrinsic and extrinsic pathway of apoptosis. In addition, CTSD secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogenous inhibitor of angiogenesis, suggesting its contribution to the prevention of tumor growth and angiogenesis-dependent growth of metastases. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Mouse Cathepsin D / CTSD Protein (TMPY-00733) |
| Antigen Species | Mouse |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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