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Anti-Phospho-CD130/gp130 (Ser782) Polyclonal Antibody
Catalog No. TMAB-10449 Copy Product Info
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Anti-Phospho-CD130/gp130 (Ser782) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CD130/gp130 (Ser782). Anti-Phospho-CD130/gp130 (Ser782) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Anti-Phospho-CD130/gp130 (Ser782) Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-10449
Anti-Phospho-CD130/gp130 (Ser782) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CD130/gp130 (Ser782). Anti-Phospho-CD130/gp130 (Ser782) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Anti-Phospho-CD130/gp130
(Ser782) Polyclonal Antibody
(Ser782) Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Bioactivity
Antigen Details
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| Description | Anti-Phospho-CD130/gp130 (Ser782) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CD130/gp130 (Ser782). Anti-Phospho-CD130/gp130 (Ser782) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
| Ig Type | IgG |
| Reactivity | Human,Rat,Rabbit (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse) |
| Verified Activity | 1. phosphopeptide non phosphopeptide 2. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-gp130 (Ser782)) Polyclonal Antibody, Unconjugated (TMAB-10449) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-gp130 (Ser782)) Polyclonal Antibody, Unconjugated (TMAB-10449) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 4. Sample: Lane 1: Human HepG2 cell Lysates Lane 2: Human HUVEC cell Lysates Primary: Anti-phospho-CD130gp130 (Ser782) (TMAB-10449) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kDa Observed band size: 130 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. |
| Tissue Specificity | Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | bs-1459P is one synthetic peptide derived from human IL6R beta. CD130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. |
| Immunogen | KLH conjugated synthesised phosphopeptide: human gp130 around the phosphorylation site of Ser782 |
| Antigen Species | Human |
| Gene Name | IL6ST |
| Gene ID | |
| Protein Name | Interleukin-6 receptor subunit beta |
| Uniprot ID | |
| Function | Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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