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Anti-ADAM15 Antibody (5X82)
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Catalog No. TMAY-00937
Alias metargidin, MDC15, ADAM metallopeptidase domain 15
Anti-ADAM15 Antibody (5X82) is a Rabbit antibody targeting ADAM15. Anti-ADAM15 Antibody (5X82) can be used in ELISA,ELISA(Det),IHC-P,FCM,ICC/IF.
Anti-ADAM15 Antibody (5X82)
Anti-ADAM15 Antibody (5X82)
😃Good
Catalog No. TMAY-00937Alias metargidin, MDC15, ADAM metallopeptidase domain 15
Anti-ADAM15 Antibody (5X82) is a Rabbit antibody targeting ADAM15. Anti-ADAM15 Antibody (5X82) can be used in ELISA,ELISA(Det),IHC-P,FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $94 | 7-10 days | |
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: ELISA, ELISA(Det), IHC-P, FCM, ICC/IF Reactivity: Human |
| Alias | metargidin, MDC15, ADAM metallopeptidase domain 15 |
| Ig Type | Rabbit IgG |
| Clone | 5X82 |
| Reactivity | Human |
| Verified Activity | 1. Profile of anti-ADAM15 reactivity on MCF-7 cells analyzed by flow cytometry. 2. Immunohistochemical staining of formalin fixed, paraffin-embedded human lymphonode showing membrane staining of endothelial cells (red arrow) (1:2000). 3. Immunohistochemical staining of formalin fixed, paraffin-embedded human heart showing blood vessel staining (red arrow) (1:200). 4. Immunofluorescence staining of Human ADAM15 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ADAM15 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm. |
| Application | ELISA,ELISA(Det),IHC-P,FCM,ICC/IF |
| Recommended Dose | ELISA: 1:5000-1:10000; IHC-P: 1:1000-1:4000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100; ELISA(Det): 1:1000-1:10000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human ADAM15 (rh ADAM15; TMPY-00961; NP_997074.1; Met 1-Thr 696). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | ADAM15, also known as Metargidin, is a type I transmembrane glycoprotein belonging to the ADAM (A Disintegrin and Metalloprotease Domain) family of proteins and is widely expressed in different tissues and cell types. Members of this family contain an amino-terminal metalloprotease domain followed by a disintegrin domain, a cysteine-rich region and a membrane proximal EGF-like domain. The disintegrin domain of ADAM15/metargidin contains an RGD tripeptide sequence, suggesting that it may potentially interact with the integrin family of proteins. ADAM15 is a transmembrane multi-domain proteins implicated in proteolysis, cell-cell and cell-matrix interactions in various disease conditions. There is also evidence supporting a role for ADAM15 in angiogenesis and angioinvasion of tumor cells, which are critical for unrestrained tumor growth and metastatic spread. Given its diverse functions, ADAM15 may represent a pivotal regulatory component of tumor progression, an important target for therapeutic intervention, or emerge as a biomarker of disease progression. |
| Conjucates | Unconjugated |
| Others Formats | APC/PE |
| Antibody Types Available | 3 |
| Immunogen | Recombinant Protein: Human ADAM15 protein (TMPY-00961) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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