Anti-CD200R Polyclonal Antibody 2 is a Rabbit antibody targeting CD200R. Anti-CD200R Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

IgG

Human

1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-CD200R Polyclonal Antibody, Unconjugated (TMAB-03910) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
A549 (Human) Cell Lysate at 30 μg
Primary: Anti-CD200R (TMAB-03910) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
3. Sample:
K562 (Human) Cell Lysate at 30 μg
Primary: Anti-CD200R (TMAB-03910) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
4. Measured by its binding ability in a indirect ELISA. Immobilized Mycobacterium bovis protein at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-CD200R Antibody, the EC50 is 158 ng/mL.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: human CD200R

Human

CD200R1

Cell surface glycoprotein CD200 receptor 1

Receptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.