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Anti-VP16 tag Polyclonal Antibody
Catalog No. TMAB-14126 Copy Product Info
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Anti-VP16 tag Polyclonal Antibody is a Rabbit antibody targeting VP16 tag. Anti-VP16 tag Polyclonal Antibody can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.
Anti-VP16 tag Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-14126
Anti-VP16 tag Polyclonal Antibody is a Rabbit antibody targeting VP16 tag. Anti-VP16 tag Polyclonal Antibody can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.
Anti-VP16 tag Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
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| Description | Anti-VP16 tag Polyclonal Antibody is a Rabbit antibody targeting VP16 tag. Anti-VP16 tag Polyclonal Antibody can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB. |
| Ig Type | IgG |
| Reactivity | Species independent |
| Verified Activity | Sample: Lane 1: VP16-Tagged Fusion Protein Overexpression E. Coli Lysate at 2 μg Lane 2: VP16-Tagged Fusion Protein Overexpression E. Coli Lysate at 4 μg Primary: Anti-VP16 tag (TMAB-14126) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD |
| Application | |
| Recommended Dose | ELISA=1:1000-5000; FCM=1 μg/Test; ICC/IF=1:100-500; IF=1:200-1000; IHC-Fr=1:200-1000; IHC-P=1:200-1000; WB=1:1000-5000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | HSV evades the immune system through interference with MHC class I antigen presentation on the cell surface, by blocking TAP or the transporter associated with antigen processing induced by the secretion of ICP-47 by HSV. In the host cell, TAP transports digested viral antigen epitope peptides from the cytosol to the endoplasmic reticulum, allowing these epitopes to be combined with MHC class I molecules and presented on the surface of the cell. Viral epitope presentation with MHC class I is a requirement for activation of cytotoxic T-lymphocytes (CTLs), the major effectors of the cell-mediated immune response against virally-infected cells. ICP-47 prevents initiation of a CTL-response against HSV, allowing the virus to survive for a protracted period in the host. |
| Immunogen | KLH conjugated synthetic peptide: HSV-1 VP16 |
| Gene Name | UL48 |
| Protein Name | Tegument protein VP16 |
| Function | Transcriptional activator of immediate-early (IE) gene products (alpha genes). Acts as a key activator of lytic infection by initiating the lytic program through the assembly of the transcriptional regulatory VP16-induced complex composed of VP16 and two cellular factors, HCFC1 and POU2F 1. VP16-induced complex represents a regulatory switch: when it is on, it promotes IE-gene expression and thus lytic infection, and when it is off, it limits IE-gene transcription favoring latent infection (By similarity).
May play a role in the aggregation of tegument proteins around nucleocapsids during virus morphogenesis. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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