EFNB2A Protein, Danio rerio (zebrafish), Recombinant (His)

Name: EFNB2A Protein, Danio rerio (zebrafish), Recombinant (His)
Brand: TargetMol
SKU: TMPY-03633
Rating: 4.5 (1 reviews)

(Synonyms: id:ibd572, EFNB2A, efnb2) Copy Product Info

Synonyms: id:ibd572, EFNB2A, efnb2

Catalog No. TMPY-03633 Copy Product Info

EFNB2A Protein, Danio rerio (zebrafish), Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.3 kDa and the accession number is O73874.

Pack Size	Price	USA Stock	Global Stock
5 µg	$48	7-10 days	7-10 days
10 µg	$77	7-10 days	7-10 days
20 µg	$123	7-10 days	7-10 days
50 µg	$242	7-10 days	7-10 days
100 µg	$397	7-10 days	7-10 days
200 µg	$790	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized zebrafish EFNB2A-His at 10 μg/ml (100 μl/well) can bind human EphB4-Fc , The EC50 of human EphB4-Fc is 15.5-36.3 ng/ml.
Description	EFNB2A Protein, Danio rerio (zebrafish), Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.3 kDa and the accession number is O73874.
Species	Danio rerio (zebrafish)
Expression System	HEK293 Cells
Tag	C-His
Accession Number	O73874
Construction	A DNA sequence encoding the zebrafish EFNB2A (O73874) (Met 1-Ala222) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Leu 25
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, PH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	id:ibd572, EFNB2A, efnb2
Research Background	Efnb2a, also known as ephrin B2a, belongs to the ephrin family. Members of this family are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Efnb2a binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Efnb2a, together with ephb4 may play a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration.

Chemical Properties

Molecular Weight

23.3 kDa (predicted); 35-40 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: EFNB2A Protein, Danio rerio (zebrafish), Recombinant (His) chemical structure | EFNB2A Protein, Danio rerio (zebrafish), Recombinant (His) molecular weight