Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IHC, IF

Reactivity: Human

Rabbit IgG

6T865

Human

1. Western Blot
-Positive WB detected in: 293 whole cell lysate(20µg), K562 whole cell lysate(20µg), Hela whole cell lysate(20µg), MCF-7 whole cell lysate(20µg), PC-3 whole cell lysate(20µg)
-All lanes: CYP19A1 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 58, 25 kDa
-Observed band size: 58 kDa
-Exposure time: 120s
2. IHC image of TMAH-00327 diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
3. Immunofluorescence staining of Hela cell with TMAH-00327 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
4. Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ELISA, WB, IHC, IF

WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human CYP19A1

Human

1588

Cell Biology