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Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9E427)

🥰Excellent
Catalog No. TMAY-02873

Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9E427) is a Chimera antibody targeting SARS-CoV/SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9E427) can be used in WB,ELISA,IHC-P,ICC/IF.

Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9E427)

Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9E427)

🥰Excellent
Catalog No. TMAY-02873
Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9E427) is a Chimera antibody targeting SARS-CoV/SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9E427) can be used in WB,ELISA,IHC-P,ICC/IF.
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Product Introduction

Bioactivity
Description
Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9E427) is a Chimera antibody targeting SARS-CoV/SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody (9E427) can be used in WB,ELISA,IHC-P,ICC/IF.
Ig Type
Monoclonal Mouse (variable region) / human (kappa / IgG1 constant) chimeric antibody
Clone
9E427
Verified Activity
1. Anti-SARS-CoV / SARS-CoV-2 Nucleocapsid Chimeric monoclonal antibody at 1:2000 dilution.

-Lane A: SARS-COV-2 Nucleocapsid (YP_009724397.2, WT) overexpressed HEK293 Whole Cell Lysate.

-Lane B: HEK293 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Human IgG Fc/HRP at 1/1000 dilution.

-Developed using the ECL technique.

-Performed under reducing conditions. (Validation Experiment)

2. Immunochemical analysis of SARS-COV-2 Nucleocapsid (YP_009724397.2, WT) overexpressed HEK293 Cells were stained with purified anti-SARS Nucleocapsid Chimera Mab,then a HRP-conjugated second step antibody. (Validation Experiment)

3. Immunofluorescence analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS Nucleocapsid Chimera Mab,then a FITC-conjugated second step antibody. (Validation Experiment)
Application
WB,ELISA,IHC-P,ICC/IF
Recommended Dose
WB: 1:2000-1:5000; ELISA: 1:5000-1:10000; IHC-P: 1:20-1:100; ICC-IF: 1:20-1:100
Antibody Type
Monoclonal
Host SpeciesChimera
ConstructionIt is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions, and the variable region was from mouse monoclonal antibody (Cat#40143-MM08). The chimeric monoclonal antibody was produced using recombinant antibody technology.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundCoronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant SARS-CoV Nucleoprotein / NP Protein (TMPY-02870)
Antigen Species
SARS
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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