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Anti-Vimentin Antibody (6E514)
😃Good
Catalog No. TMAY-00108
Alias vimentin, HEL113, CTRCT30
Anti-Vimentin Antibody (6E514) is a Rabbit antibody targeting Vimentin. Anti-Vimentin Antibody (6E514) can be used in ELISA,ICC/IF.
Anti-Vimentin Antibody (6E514)
Anti-Vimentin Antibody (6E514)
😃Good
Catalog No. TMAY-00108Alias vimentin, HEL113, CTRCT30
Anti-Vimentin Antibody (6E514) is a Rabbit antibody targeting Vimentin. Anti-Vimentin Antibody (6E514) can be used in ELISA,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: ELISA, ICC/IF Reactivity: Human |
| Alias | vimentin, HEL113, CTRCT30 |
| Ig Type | Rabbit IgG |
| Clone | 6E514 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of VIM in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human VIM monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm. |
| Application | ELISA,ICC/IF |
| Recommended Dose | ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Vimentin (rh Vimentin; TMPY-04329; P08670; Met1-Glu466). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Vimentin is a type III intermediate filament (IF) protein found in various non-epithelial cells, especially mesenchymal cells. A vimentin monomer, has a central α-helical domain and carboxyl (tail) domains. Two monomers compose the basic subunit of vimentin assembly. Vimentin is crucial for supporting and anchoring the position of the organelles in the cytosol. Vimentin provided cells with a resilience absent from the microtubule or actin filament networks, when under mechanical stress in vivo. Therefore, in general, it is accepted that vimentin is the cytoskeletal component responsible for maintaining cell integrity. Vimentin is also responsible for stabilizing cytoskeletal interactions. It is found that vimentin control the transport of low-density lipoprotein. It has been used as a sarcoma tumor marker to identify mesenchyme. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human Vimentin Protein (TMPY-04329) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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