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Anti-14-3-3 beta Antibody (7G975)
🥰Excellent
Catalog No. TMAY-03881
Anti-14-3-3 beta Antibody (7G975) is a Mouse antibody targeting 14-3-3 beta. Anti-14-3-3 beta Antibody (7G975) can be used in WB,ELISA,IP.
Anti-14-3-3 beta Antibody (7G975)
Anti-14-3-3 beta Antibody (7G975)
🥰Excellent
Catalog No. TMAY-03881
Anti-14-3-3 beta Antibody (7G975) is a Mouse antibody targeting 14-3-3 beta. Anti-14-3-3 beta Antibody (7G975) can be used in WB,ELISA,IP.
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Product Introduction
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Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-14-3-3 beta Antibody (7G975) is a Mouse antibody targeting 14-3-3 beta. Anti-14-3-3 beta Antibody (7G975) can be used in WB,ELISA,IP. |
| Ig Type | Monoclonal Mouse IgG2b |
| Clone | 7G975 |
| Reactivity | Cynomolgus |
| Verified Activity | 1. Anti-YWHAB mouse monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:28 kDa. -Observed band size:32 kDa. 2. cyno YWHAB was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -Lane B:0.5 mg Hela Whole Cell Lysate. -2 µL anti-cyno YWHAB mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-cyno YWHAB mouse monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 32 kDa. -Observed band size: 32 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IP: 4-6 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Cynomolgus 14-3-3 beta / YWHAB (TMPY-02584; Q4R572-2; Met 2-Asn 244). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | 14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Cynomolgus 14-3-3 beta / YWHAB protein (TMPY-02584) |
| Antigen Species | Cynomolgus |
| Biology Area | phospho-Serine/phospho-Threonine Binding Proteins, Adaptor Proteins, Apoptosis Adaptor Proteins |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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