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CD3D & CD3E Heterodimer Protein, Cynomolgus, Recombinant (His & Flag)
(Synonyms: T3D, CD3E&CD3D, CD3e, CD3E, CD3d, CD3D, CD3 delta&CD3 epsilon, CD3) Copy Product InfoSynonyms: T3D, CD3E&CD3D, CD3e, CD3E, CD3d, CD3D, CD3 delta&CD3 epsilon, CD3
Catalog No. TMPK-01625 Copy Product Info
CD3D & CD3E Heterodimer Protein, Cynomolgus, Recombinant (His & Flag) is expressed in HEK293 Cells. The accession number is Q95LI5(CD3E)&Q95LI8(CD3D).
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Cynomolgus CD3E&CD3D, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 7.2 ng/ml determined by ELISA (QC Test). |
| Description | CD3D & CD3E Heterodimer Protein, Cynomolgus, Recombinant (His & Flag) is expressed in HEK293 Cells. The accession number is Q95LI5(CD3E)&Q95LI8(CD3D). |
| Species | Cynomolgus |
| Expression System | HEK293 Cells |
| Tag | C-His, C-Flag |
| Accession Number | Q95LI5 (CD3E) & Q95LI8 (CD3D) |
| Construction | Gln22-Asp117 (CD3E) and Phe22-Ala105 (CD3D) |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Endotoxin | < 0.1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | T3D, CD3E&CD3D, CD3e, CD3E, CD3d, CD3D, CD3 delta&CD3 epsilon, CD3 |
| Research Background |
| Molecular Weight | 16.17 kDa (CD3E) and 15.01 kDa (CD3D) (Predicted); 17-23 kDa and 35-45 kDa (Reducing condition) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: CD3D & CD3E Heterodimer Protein, Cynomolgus, Recombinant (His & Flag) chemical structure | CD3D & CD3E Heterodimer Protein, Cynomolgus, Recombinant (His & Flag) molecular weight
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