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Anti-ICAM-1/CD54 Antibody (4H175)
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Catalog No. TMAY-00663
Alias P3.58, intercellular adhesion molecule 1, ICAM-1, CD54, BB2
Anti-ICAM-1/CD54 Antibody (4H175) is a Mouse antibody targeting ICAM-1/CD54. Anti-ICAM-1/CD54 Antibody (4H175) can be used in ELISA,ELISA(Cap),FCM,ICC/IF.
Anti-ICAM-1/CD54 Antibody (4H175)
Anti-ICAM-1/CD54 Antibody (4H175)
😃Good
Catalog No. TMAY-00663Alias P3.58, intercellular adhesion molecule 1, ICAM-1, CD54, BB2
Anti-ICAM-1/CD54 Antibody (4H175) is a Mouse antibody targeting ICAM-1/CD54. Anti-ICAM-1/CD54 Antibody (4H175) can be used in ELISA,ELISA(Cap),FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $69 | 7-10 days | |
| 100 μL | $198 | 7-10 days |
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Chemical Properties
| Description | Anti-ICAM-1/CD54 Antibody (4H175) is a Mouse antibody targeting ICAM-1/CD54. Anti-ICAM-1/CD54 Antibody (4H175) can be used in ELISA,ELISA(Cap),FCM,ICC/IF. |
| Alias | P3.58, intercellular adhesion molecule 1, ICAM-1, CD54, BB2 |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 4H175 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human ICAM1 in MSC cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with Mouse anti-Human ICAM1 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane. 2. Flow cytometric analysis of Human ICAM1 (CD54) in HUVEC cells. HUVEC cells (Human umbilical vein endothelial cells) were either treated with TNF-α (50ng/ml,18 hours) (right panel) or untreated (left panel). The cells were harvested and stained with purified human ICAM1, then a FITC-conjugated second step antibody. |
| Application | ELISA,ELISA(Cap),FCM,ICC/IF |
| Recommended Dose | ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100; ELISA(Cap): 1:250-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ICAM1 / CD54 (rh ICAM1 / CD54; TMPY-01142; Met 1-Glu 480; NP_000192.2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. ved. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity. |
| Conjucates | Unconjugated |
| Others Formats | APC/PerCP/FITC/PE |
| Antibody Types Available | 5 |
| Immunogen | Recombinant Human ICAM-1 protein (TMPY-01142) |
| Antigen Species | Human |
| Biology Area | Neuroinflammation |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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